DETERMINATION OF IRON CONCENTRATION IN BLOOD USING A CHEMILUMINESCENCE METHOD WITH A MOBILE PHONE-BASED DETECTOR

Abstract

This research focuses on developing a novel chemiluminescence (CL) detection device for measuring Fe(III)  concentration using a mobile phone-based system. The study addresses the need for a more sensitive and cost-effective method to detect iron (III), particularly in the blood of Thalassemia patients. The device utilizes a photosensor with high sensitivity, a simple design, and user-friendly operation. The experimental section details developing the device, which measures light intensity generated from the reaction between Luminol and hydrogen peroxide in the presence of iron (III) as a catalyst.  Optimal reaction conditions were established to ensure accurate measurements. Light intensity was detected using a mobile phone equipped with the Remote XY application, which converted the signal into millivolts (mV) for analysis. The calibration curve followed Beer's law within a concentration range of 1×10⁻⁵ to 1×10⁻¹ M, with a molar absorption coefficient (Ɛ) of 2.09 × 10⁵ L·mol⁻¹·cm⁻¹. The limit of detection (LOD) was determined to be 0.21 ppm, and the limit of quantification (LOQ) was 0.7 ppm, with a correlation coefficient (R²) of 0.9997 and a relative standard deviation (RSD%) of 0.082%. The device was tested on 25 blood samples from Thalassemia patients, and the results were validated against a colorimetric Cromatest kit, yielding T-test and F-test values of 1 and 0.976, respectively, indicating strong agreement between the two methods.