COMPARISON OF LD50 OF BENZOIC ACID, METHYLPARABEN, AND A COMBINATION OF BOTH BY USING YEAST MODEL

Abstract

Background: Preservatives are chemicals, either natural or artificial, that are frequently added to a variety of foods and medicinal goods to stop microbial development and breakdown. Method: In this study, we compared the LD₅₀ which gives the idea of which chemical or preservative is more toxic to the human body. Yeast culture was prepared in 4% (w/v) dextrose solution and incubated for 48 hrs. Different concentrations of preservatives were prepared in a yeast and dextrose NaCl solution by serial dilution. Each of the concentrations was plated and incubated for 2x48 hrs. The data obtained from Image analysis were interpreted with the help of graphs. Result: On comparison of the LD₅₀ of Benzoic acid and Methylparaben, no significant difference was found at 48 hours and 96 hours. Whereas, contrast was obtained on comparison of LD₅₀ of Methylparaben and a combination of both as well as Benzoic acid and a combination of both by p-value less than 0.001 & 0.01 (48 hrs). Conclusion: Hence, it was concluded that the combined form of benzoic acid and methylparaben, when used at a low concentration of 0.1% was very effective. However, it contains a high LD₅₀ as compared to individual Methylparaben and Benzoic acid, which was used at high concentrations of 0.2 %.