ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM MICHELIA CHAMPACA LINN. WITH POTENTIAL ANTI-ARTHRITIC ACTIVITY

Abstract

The flowers of Michelia champaca .L have been reported to possess certain benzo quinoline alkaloids. Thus, this study was aimed at the anti-arthritic potential and phytochemical screening of two important compounds CMPK-1, tetrahydro benzodioxolo benzo quinolin and CMPK-2, tetrahydro benzodioxolo benzo quinolin-16-ol (alkaloids) of flowers of Michelia champaca from with nHexane fractions of petroleum ether extract. Isolated alkaloids was identified and characterized by chemical tests, M.P., TLC, paper chromatography and spectroscopic methods like UV, Visible, FT-IR, ¹HNMR, ¹³CNMR and Mass spectroscopy. The anti-arthritic activity of the isolated alkaloids were also investigated against standard Diclofenac sodium by Carrageenan induced arthritic in rats. The results demonstrated shows significant anti-arthritic activity of isolated compounds at a dose of 500 mg/kg body weight might prove efficacious for further design and development of agents with significant biological activity. Hence, isolation was done with petroleum extract. Isolated alkaloids CMPK-1 and CMPK-2 may be one of the components responsible for the observed anti-microbial activity of the flowers.