Concentration of Symphytum officinale extracts with cytostatic activity by tangential flow ultrafiltration
Keywords:
Symphytum officinale, ultrafiltration, cytostatic activity, HeLa cells, potential antitumoral agentsAbstract
Symphytum officinale L (Boraginaceae) species are currently used in the Romanian traditional medicine to treat different human and animal disease, being also active in certain cancer forms.
This work’s aim consists in obtaining of Symphytum officinale concentrated extracts by using performance membrane processes, aqueous extracts prepared were concentrated by tangential flow ultrafiltration with a Koch Laboratory Cell CF-1 membrane. The cytostatic activity of the total plant extracts were studied on HeLa cells culture. By comparison to the bystander value of 100%, we noticed that in vitro treatment of HeLa neoplasic cells with these concentrated extracts determined a mitoinhibitory effect with statistical and cytostatical significant amplitude. These values were of almost 57,6% for Symphytum officinale.
