Original bioactive complexes rich in glycosaminoglycans obtained from small fish

Abstract

An  original technology was  developed to  obtain  a  bioactive complex from small fish  species Engraulis encrassicolus ponticus, Sprattus sprattus sprattus, Odontogadus merlangus euxinus) which contains:  glycosaminoglycans,  essential  amino  acids,  essential  fatty  acids  (linoleic,  linolenic  and arachidonic acids), eicosanoids, vitamins (myoinositol), glycerolphosphates, microelements: Ca, Na, K, Fe, Mg, Se, Ni, Cu, Si, etc. The bioactive complex was analyzed by gas chromatography coupled with  mass­spectrometry,  gel  electrophoresis  in  different  systems  (SDS­poliacrilamide,  cellulose acetate),  potentiometric  titration,  and  high  performance  liquid  chromatography.  The  content  of glycosaminoglycans  (from  which  44%  represent  sulfated glycosaminoglycans)  make  the  bioactive complex  useful  to  prevent  the  un  unsettlement  of  the  macromolecular  structure  of  background substance and keep the functionality of the extracellular matrix from conjunctive, cartilaginous and bone tissue. Antioxidant  activity  and  regenerative  of collagen fibrils was investigated in vitro. The chemical  composition  provides  multiple  therapeutically  properties:  anticoagulant,  antithrombotic, antilipidemic, tissue restoration, a strong antioxidant and anti­inflammatory activities. The complex obtained  is  rich  in  biological  active  compounds  and  could  be  used  to  make  pharmaceutical preparations with high efficiency and minimal side effects.