The comparison of persimmon genotypes (Diospyros kaki Thunb.) by using RAPD and FAME data

Abstract

In  the  current  study,  FAME  (Fatty  acid  methyl  esters)  and  RAPD  profiles  were  used  to examine  biochemical  and  genetic  relationships  between  31  selected  persimmon  genotypes  sampled from Persimmon Repository, Black Sea Agricultural Research  Institute, Samsun, Turkey. Fatty acid composition  of  persimmon leaves was  determined  by  using  gas  chromatography.  The  dendrogram realized  from  the  RAPD  markers  grouped  the  31  genotypes  into  three  major  clusters.  Cluster  1 included 8, cluster 2 included 14 and cluster 3 included 9 genotypes. The fatty acid results showed that there were differences among persimmon genotypes both percent and presence of fatty acids in leaves.  Some  relationships  between  RAPD  and  FAME  data  were  found.  The  linolenic  acid  was detected only in genotypes in cluster 3. Behenic acid was detected only in genotypes within cluster 1. According to these results, it can be concluded that the use of RAPD and FAME data could be useful indicator for characterization and grouping of persimmon genotypes.