Lipase Production in Discontinuous Operation System Using a Candida lipolytica Strain

Abstract

The optimal growth conditions for a Candida lipolytica strain and an increased lipase production in a batch culture system have been determined, by gradually increasing the impeller speed (400 – 600 – 800 – 900 rpm) and the aeration (2.0 – 2.5 – 3.0 – 4.0 L/min) during the bioprocess time . 
The lipolytic activities into the broth reached maximum levels within 20 - 22 h after inoculation, when the microorganisms were at the beginning of the stationary growth phase.