Microbial Enzymes-Lectins Interactions: Applications for Glycoproteins Purification

Abstract

Bacteria from Bacillus species produce a large variety of extracellular 
enzymes, a lot of them providing biotechnological importance (a complex enzymeproduct including α-amylase, protease, β-glucanases, necessary in beer industry is obtained in the culture media from Bacillus subtilis strains). Filamentous fungi, such as those belonging to Trichoderma reesei, produce a potent cellulosolytic enzymes–set able to achieve efficient hydrolysis of a highly crystallized cellulose and consequently of natural cellulose forms degradation resisting. This work present some experimental studies based on interaction between plant lectins and components of cellulosolytic systems produced by strains of Bacillus subtilis and Trichoderma reesei useful to improve the enzymes purification.