Analytical Measurements in Metabolic Changes of Bacterial Medium

Abstract

Continuous monitoring of a bacterial culture for pH, growth CO2 and NH3 in 
microbiological medium. Changes in the metabolic parameters of Proteus sp. cultures generally occurred about one or two hours before changes in growth were observed. The time of maximum CO2 production preceded that of NH3 elaboration by this organism. This type of monitoring system has great potential for the study of the metabolism of growing organisms as well as for the early detection of growth in liquid culture. Studies of the protozoan organism have suggested that the major source of substrate for the growth and energy production of the organism may be amino acids rather than carbohydrates. In such studies of the metabolism of microorganism, frequent determinations are necessary and the usual technique requires periodic sampling of the material under study.