Alcohol Dehydrogenase Activity in Carassius carassius White Skeletal Muscle

Authors

  • D. DINU, I. F. DUMITRU, M. T. NECHIFOR Author

Keywords:

alcohol dehydrogenase, Carassius carassius, enzyme purification, Km values

Abstract

A form of alcohol dehydrogenase was prepared from white skeletal muscle of Carassius carassius by acetone precipitation, Sephacryl S-200 gel filtration and DEAE-Fractogel anion-exchange chromatography. We have determined the molecular weight and the kinetic properties of this enzyme. The molecular weight value, 81,400 Da, was very similar to mammalian alcohol dehydrogenase. The high Km value for ethanol (555 x 10-1 M) suggested that this enzyme might be a class III alcohol dehydrogenase. 

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Published

2023-09-27

Issue

Vol. 41 No. 9 (2023)

Section

Articles