Using of unconventional methods for obtaining somaclonal variations, having as goal making of new potato varieties with resistance at diseasesand pests

Abstract

The most common factors which affect somaclonal variations are genotype explant source, in vitro period and cultivation conditions in which the culture is established. In this research, calli were induced  using leaf disk and leafstalks explants of potato cultivars resistant at potato blight and viroses. Calli were induced with dif erent growing regulators (with dif erent concentrations). Having as objective obtaining of lines with resistance at diseases and pest are necessary: callus induction, callus growing, callus multiplication  by subculture, controlling  organogenesis processes. Plantlets regeneration from callus is influenced by genotype ad medium variant.