Cellulosic materials functionalized with reactive dyes and metallic ions for biotechnologal separations

Abstract

Affinity chromatography is a valuable method for the isolation of a specific biomolecule from complex mixtures in biotechnological processes. Affinity chromatography is a separation technique based on the use of specific and selective immobilized ligands able to associate reversibly to a desired biomolecule. New celluloses materials functionalized with reactive dyes and metallic ions are of interest in affinity separation of enzymes. This paper presents a sorption study regarding the testing the retained capacity of the microcrystalline aminoethyl (AE) and guanidoethyl (GE) celluloses modified with reactive dye Brilliant Red HE ñ 3B and Cu2+ ions, for the lysozyme molecules from aqueous solutions.