The release of l­asparaginase from hydrogel­magnetic nanoparticles

Abstract

L­asparaginase was immobilized by entrapment in hydrogel­magnetic nanoparticles obtained from  magnetic  nanoparticles  and  successive  layers  of  chitosan  and  hyaluronic  acid.  The  obtained nanostructures,  with  dimensions  suitable  for  penetration  cell/tissues,  especially  tumor tissues,  were evaluated for  swelling  behavior,  degradation  behavior  and the  release  of  L­asparaginase in  neutral media and slight acid media  (pH 4.5). The experiments showed a good swelling behavior for all the tested  samples,  but  a  high  stability  of  nanostructures.  L­asparaginase  has  an  initial  burst  from hydrogel, at two hours, and other two releases at 24 and between 50 and 72 hours.