Characteristics of RAPD markers in breeding of Cucumis sativus L

Abstract

The  goal  of this  research was to  assess  genetic  polymorphism  of  RAPD fragments that  are relevant  to  their  use  in  the  genetic  analysis  of  cucumber  genotypes.  It  was  discussed  potential application of RAPD primers in genotype classification by cluster analysis. Besides, RAPD profiles were  determined  to  be  dominantly  and  co­dominantly  inherited  in  first  generation  hybrids.  It  was possible to distinguish some of the genotypes due to the presence of several specific DNA products. Genetic distances between parental lines, estimated on the base of RAPD patterns, showed medium (­0,34  and  0,39) and  strong  (0,81 and  0,84) correlations with  productivity  of  hybrids. As  a  result, it was  shown  the  possibility  of  implementation  in  prognosis  of  heterosis  effect  in  F1  hybrids  and  in breeding programs of Cucumis sativus L.