Genetically modified medicinal plants. II. Transfer and expression of a marker kanamycine resistance gene in Atropa belladonna plants
Keywords:
Atropa belladonna, Agrobacterium tumefaciens, transgenic plant, marker nptII gene, NPTII-protein neomycin phosphotransferase II, PCR-polymerase chain reactionAbstract
An indirect, mediated genetic transformation of Atropa belladona vitroplants
has been performed using the disarmed Agrobacterium tumefaciens pTi carrying the nptII gene for neomycinephosphotransferase. Its expression into the plant host genome conferred the kanamycin resistance trait which has been easily detected by both in vitro and molecular-genotype (PCR) and phenotype (protein) - assays.
